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Genotypic and antimicrobial resistance characterizations of Cronobacter sakazakii isolated from powdered milk infant formula: A comparison between domestic and imported products

Pakbin, Babak and Mahmoudi, Razzagh and Mousavi, Shaghayegh and Allahyari, Samaneh and Amani, Zahra and Peymani, Amir and Qajarbeygi, Peyman and Hoseinabadi, Zahra (2020) Genotypic and antimicrobial resistance characterizations of Cronobacter sakazakii isolated from powdered milk infant formula: A comparison between domestic and imported products. Food Science and Nutrition, 8. pp. 6807-17.

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Abstract

Cronobacter sakazakii, an opportunistic foodborne pathogen and a main cause of meningitis in neonates, is usually isolated from powdered milk infant formula (PMIF). At the present study, C. sakazakii were isolated from imported and domestically produced PMIF samples and identified by detection of ompA gene using real-time PCR SYBR green melting curve following the evaluation of antimicrobial susceptibility and genotyping of the isolates employing BOX-PCR and RAPD methods. We detected totally 5% contamination rate and a significantly higher prevalence of C. sakazakii in bulky imported domestically packaged PMIF samples. Also, our isolates were recognized as multidrug-resistant pathogen completely resistant to ampicillin and amoxicillin; and intermediately resistant to ciprofloxacin and tetracycline antimicrobials. Genotype clustering patterns of bulky imported and imported product isolates were identical by both genotyping methods. Far genetic relatedness of domestic isolate to other isolates and the reference strain indicated higher genetic diversity of the domestic isolate genome. Multidrug resistance and diverse population genetic make complicated situation for determination of strategies for infectious disease prevention.

Item Type: Article
Subjects: Q Science > QR Microbiology
R Medicine > RN Health > RN1005 Health Sciences in Nutrition
Divisions: University Portal > vice chancellor > vcr
Depositing User: Mrs Shahrzad Gholami
Date Deposited: 14 Dec 2020 09:58
Last Modified: 14 Dec 2020 09:58
URI: http://eprints.qums.ac.ir/id/eprint/10287

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