Rahmani, Babak and Hamedi Asl, Dariush and Naserpour Farivar, Taghi and Azad, Mehdi and Sahmani, Mehdi and Gheibi, Nematollah (2019) Omega-3 PUFA Alters the Expression Level but Not the Methylation Pattern of the WIF1 Gene Promoter in a Pancreatic Cancer Cell Line (MIA PaCa-2). Biochemical Genetics, 57 (4). pp. 477-486.
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Abstract
Pancreatic cancer is the fourth leading cause of death in both males and females, with a 5-year relative survival rate of 8%. The Wnt signaling pathway has a signifcant role in the pathogenesis of many tumors, including those of pancreatic cancer. Hypermethylation of the Wnt inhibitory Factor-1 (WIF1) gene promoter have been detected in diferent types of cancer. In contrast, the anticancer efects of long-chain omega-3 PUFA (ALA) have been reported. Regarding its anticancer efects, in this study, we investigated the efects of various concentrations of omega-3 PUFA on expression level and promoter methylation of the WIF1 gene in MIA PaCa-2 cells in 24, 48, and 72 h after treatment. MIA PaCa-2 cells were treated with diferent concentrations of omega-3 PUFA (25, 50, 100, 250, 500, and 1000 μM). Cell viability assay was carried out followed by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and methylation-specifc PCR (MSP). This investigation suggested that dietary consumption of omega-3 PUFAs (250–1000 μM) has a signifcant efect on the proliferation and WIF1 gene expression of the MIA PaCa-2 cancer cell line but no efect on the promoter methylation of this gene. Changes in promoter methylation were not observed in any of the treatments.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > RH Basic Medical Sciences > RH1001 Biochemistry & Genetics |
| Divisions: | University Portal > vice chancellor > vcr |
| Depositing User: | Mrs Shahrzad Gholami |
| Date Deposited: | 08 Mar 2021 05:25 |
| Last Modified: | 08 Mar 2021 05:25 |
| URI: | http://eprints.qums.ac.ir/id/eprint/10699 |
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