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Underexpression of hsa-mir-449 family and their promoter hypermethylation in infertile men: A case-control study

Najafipour, Reza and Momeni, Abdolmabood and Yousefipour, Farideh and Mousavi, Shaghayegh and Moghbelinejad, Sahar (2021) Underexpression of hsa-mir-449 family and their promoter hypermethylation in infertile men: A case-control study. International Journal of Reproductive BioMedicine, 19 (1). pp. 23-34.

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Abstract

Background: Post-transcriptional microRNAs (miRNAs) have a impotrant pattern in the spermatogenesis process. Objective: Study of the expression and methylation of hsa-miR-449 family in sperm samples of infertile men. Materials and Methods: In this case-control study, we recruited 74 infertile men (with asthenozoospermia, teratozoospermia, asthenoteratozoospermia, and oligoasthenoteratozoospermia) and 30 control samles. Methylation-specific PCR (MSP) method was used for methylation evaluation of hsa-miR-449 a, b, c promoter. By Real time PCR (qRT-PCR) method,we showed downregulation of hsa-miR-449 a, b, c in the sperm samples of infertile men and compared it to their fertile counterparts. Results: There was significant underexperssion, in hsa-miR-449-b in oligoasthenoteratospermic samples (p = 0.0001, F = 2.9). About the methylation pattern, infertile men showed high frequency of methylation in the promoter of hsa-miR-449 a, b, c in comparison to controls (60.8% vs 23.3%), the highest amount of methylation was observed in oligoasthenoteratospermia samples (81.2%). Conclusion: In this study, low expression and high methylation of hsa-miR-449-b were observed in infertile men in compared to control samples, which can be one of the causes of defective spermatogenesis.

Item Type: Article
Subjects: R Medicine > RH Basic Medical Sciences > RH1001 Biochemistry & Genetics
Divisions: University Portal > vice chancellor > vcr
Depositing User: Mrs Shahrzad Gholami
Date Deposited: 25 Apr 2021 06:17
Last Modified: 25 Apr 2021 06:17
URI: http://eprints.qums.ac.ir/id/eprint/10757

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