Detection of Extended-Spectrum beta-Lactamases among Acinetobacter Baumannii Isolated from Hospitals of Qazvin, Iran

Zarabadi-Pour, Mina and Peymani, Amir and Habibollah Pourzereshki, Narges and Sarookhani, Mohammad reza and Karami, Ali Akbar and Javadi, Amir (2021) Detection of Extended-Spectrum beta-Lactamases among Acinetobacter Baumannii Isolated from Hospitals of Qazvin, Iran. Ethiopian Journal of Health Sciences, 31 (2). pp. 229-236.

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BACKGROUND፡ Acinetobacter baumannii is a major contributor to nosocomial infections. Extended-spectrum ßlactamase (ESBL)-producing A. baumannii is spreading worldwide. We aimed to determine the frequency of ESBLencoding genes in clinical isolates of A. baumannii and to access their clonal relationship by repetitive extragenic palindromicPCR (rep-PCR). METHODS: In this descriptive cross-sectional study, 203 isolates of A. baumannii were collected from Qazvin hospitals. The Identification of isolates was performed by standard laboratory methods. To verify ESBL production, all isolates were screened by disk agar diffusion and confirmed by the combined disk method. Subsequently, ESBL-encoding genes were detected by PCR and sequencing. Possible clonal association of ESBL-producing isolates was evaluated using rep-PCR. RESULTS: Two hundred (98.5%) isolates showed reduced susceptibility to one of the antibiotics used in the ESBL screening test, of which 127 isolates (62.6%) produced ESBL. PCR results showed blaOXA-1 (20.5%) was the most prevalent gene followed by blaTEM-1 (20%), blaGES-1 (15.7%), blaCTX-M-15 (7.9%), and blaPER-1 (1.6%). Rep-PCR results revealed that ESBL-producing isolates belonged to clones A (85%), B (13.4%), and C (1.6%). CONCLUSION: Our study showed the significant presence of blaOXA-1, blaTEM-1, blaGES-1, blaCTX-M-15, and blaPER-1 genes in ESBLproducing A. baumannii isolates in the studied hospitals. This is the first report on the emergence of blaOXA-1 gene in these isolates in Iran. The use of comprehensive antimicrobial treatment guidelines based on laboratory data and appropriate infection control interventions are essential.

Item Type: Article
Subjects: Q Science > QR Microbiology
Divisions: University Portal > vice chancellor > vcr
Depositing User: Mrs Shahrzad Gholami
Date Deposited: 03 Aug 2021 04:19
Last Modified: 03 Aug 2021 04:19
URI: http://eprints.qums.ac.ir/id/eprint/10922

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