Genetic diversity and antibiotic resistance of Shigella spp. isolates from food products

Pakbin, Babak and Amani, Zahra and Allahyari, Samaneh and mousavi, Shaghayegh and mahmoodi, razagh and Manuel Brück, Wolfram and Peymani, Amir (2021) Genetic diversity and antibiotic resistance of Shigella spp. isolates from food products. Food Science and Nutrition.

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The emergence of multidrug-resistant Shigella is a significant threat to global public health. Limited studies have investigated the incidence, antimicrobial susceptibility, and genetic diversity of Shigella isolated from food products. Conventional culturebased, serologic, molecular, disk diffusion, PCR, and RAPD-PCR methods were used to determine the prevalence rate, phenotypic and genotypic antibiotic resistance profile, and genetic diversity of the Shigella isolates from food samples including vegetable salad, ground meat, and raw cow's milk (405 samples). The prevalence rate of Shigella in food samples was 4.44%. The incidence of S. sonnei (3.7%) was higher than that of S. flexneri (0.74%). S. dysenteriae and S. boydii were not detected in food samples examined. Also, no Shigella were recovered from raw cow's milk. This study showed that the Shigella isolates were resistant to sulfamethoxazole/trimethoprim (83.3%), amoxicillin (66.6%), streptomycin (66.6%), tetracycline (61.1%), ampicillin (50%), amoxicillin–clavulanic acid (50%), azithromycin (50%), and chloramphenicol (50%) and completely sensitive to cefoxitin, cefepime, amikacin, and gentamicin. All Shigella isolates were multidrug-resistant. We detected blaSHV resistance gene in all isolates; however, no isolate harbored blaTEM gene. RAPD-PCR categorized the Shigella isolates into five main clusters. The highest antibiotic resistance was observed in the isolates of cluster R4. The finding of this study also indicated an association between antimicrobial resistance profiles and genotyping properties of the isolates. Novel food monitoring systems, including surveillance of multidrug-resistant foodborne pathogens, especially in developing countries, are required to control the foodborne diseases. KEYWORDS antimicrobial resistance, food samples, genetic diversity, Shigella species

Item Type: Article
Subjects: Q Science > QR Microbiology
Divisions: University Portal > research center > mmrc
Depositing User: mmrc research portal
Date Deposited: 22 Dec 2021 06:13
Last Modified: 22 Dec 2021 06:13
URI: http://eprints.qums.ac.ir/id/eprint/11177

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