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First detection of mobilized colistin resistance mcr-1 gene in Escherichia coli isolated from livestock and sewage in Iran

Nikkhahi, Farhad and Robatjazi, Saeedeh and Niazadeh, Mojtaba and Javadi, Amir and Shahbazi, GH and Aris, Parisa and Marashi, Seyed Mahmoud Amin and Emam, N (2021) First detection of mobilized colistin resistance mcr-1 gene in Escherichia coli isolated from livestock and sewage in Iran. New Microbes New Infect.

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Abstract

Currently, few studies have investigated the mechanisms of resistance to colistin in Iran. The aim of this study was to investigate mcrharbouring Escherichia coli dissemination in livestock and sewage in Iran. A total of 115 samples from cows (n = 38), chickens (n = 47) and urban sewage samples (n = 30) were collected. The presence of genes including mcr1–6 and ampC β-lactamase (blaMOX, blaCIT, blaDHA, blaACC, blaEBC, blaFOX) for colistin-resistant isolates was investigated by multiplex PCR method. Genetic association of colistinresistant strains was also evaluated by ERIC PCR. Sixty-five isolates were identified as E. coli. Meaningless were resistant to colistin. The highest (26.1%) and lowest (3.07%) resistance were shown to ampicillin and meropenem respectively. Among the three colistin-resistant isolates, 2 (66%) were multidrug resistant, with one of them being mcr-1 positive and the other one positive for DHA ampC β-lactamase gene. No mcr2–6 genes were found. Minimum inhibitory concentration of mcr-producing isolate was 4 mg/L by microbroth dilution. This study reports, first the detection of mcr-1 in E. coli from farm animals in Iran, a finding that is indicative of a global distribution of this plasmidic element and threatning the use of colistin as a last resort antibiotic. No clonal relationship was observed between the colistinresistant E. coli isolates by ERIC-PCR. Monitoring the presence of these strains in animal sources help as to controlling the spread of resistance genes from animal to human is vital. © 2021 The Authors. Published by Elsevier Ltd.

Item Type: Article
Subjects: Q Science > QR Microbiology
Divisions: University Portal > research center > mmrc
Depositing User: mmrc research portal
Date Deposited: 11 Jan 2022 07:57
Last Modified: 11 Jan 2022 07:57
URI: http://eprints.qums.ac.ir/id/eprint/11264

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