qums

Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model

Soltan Dallal, Mohammad Mehdi and Nikkhahi, Farhad and Imeni, Seyed Mostafa and Molaei, Saber and Hosseini, Seyed Kazem and Kalaf, Zohreh and Sharifi Yazdi, Sara and Agha Mirzaei, Hedroosha Molla (2021) Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model. Journal of Ophthalmic and Vision Research.

[img] PDF
Download (115kB)

Abstract

Purpose: The use of amniotic membrane has been suggested in the treatment of infectious keratitis for its intrinsic anti-infective properties probably mediated by its antiinflammatory effects. The aim of this study was to investigate the effect of amniotic membrane transplantation (AMT) along with ciprofloxacin to cure the primary stages of Pseudomonas keratitis. Methods: In total, 28 rabbits were selected and divided in four groups as follows: group 1 as control, group 2 with amniotic membrane, group 3 with ciprofloxacin, and group 4 with amniotic membrane combined with ciprofloxacin. About 0.05 cc suspension of Pseudomonas aeruginosa, 27853 ATCC was injected into corneal stroma. Results: The results showed groups of AMT, AMT + ciprofloxacin, and ciprofloxacin had 0% perforation while the control group had 85.6%. Average infiltration of 5.5 mm was observed in ciprofloxacin group, 5 mm in AMT + ciprofloxacin group, 24 mm in AMT group, and finally 23.75 mm for control. Amniotic membrane showed to be effective in prevention of cornea perforation as well as remission of Pseudomonas keratitis. There was no significant difference between ciprofloxacin groups in comparison with ciprofloxacin + AMT group. However, regarding the anti-inflammatory effect, the process of improvement of inflammation in ciprofloxacin + AMT group was faster. Conclusion: Transplantation of amniotic membrane in the primary stages of Pseudomonas keratitis treatment remarkably prevents the disease and it can be used to control its process. Keywords: Ciprofloxacin; Human Amniotic Membrane; Keratitis; Pseudomonas aeruginosa; Rabbit

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Divisions: University Portal > research center > mmrc
Depositing User: mmrc research portal
Date Deposited: 06 Apr 2022 06:01
Last Modified: 06 Apr 2022 06:01
URI: http://eprints.qums.ac.ir/id/eprint/11434

Actions (login required)

View Item View Item
EPrints Logo
qums is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.