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Phenotype and genetic determination of resistance to common disinfectants among bioflm-producing and non-producing Pseudomonas aeruginosa strains from clinical specimens in Iran

Bakht, Mehdi and Alizadeh, Safar Ali and Rahimi, Sara and Kazemzadeh Anari, Raana and Rostamani, Mohammad and Javadi, Amir and Peymani, Amir and Marashi, Seyed Mahmoud Amin and Nikkhahi, Farhad (2022) Phenotype and genetic determination of resistance to common disinfectants among bioflm-producing and non-producing Pseudomonas aeruginosa strains from clinical specimens in Iran. BMC Microbiology.

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Abstract

Background: Pseudomonas aeruginosa is a common pathogen in Hospitalized patients, and its various resistance mechanisms contribute to patient morbidity and mortality. The main aims of the present study were to assess the susceptibility of bioflm-producing and non-producing P. aeruginosa isolates to the fve commonly used Hospital disinfectants, to evaluate the synergistic efect of selected disinfectants and Ethylene-diamine-tetra acetic acid (EDTA), and the efect of exposure to sub-inhibitory concentrations of Sodium hypochlorite on antimicrobial susceptibility test. Results: The results showed that sodium hypochlorite 5% and Ethanol 70% were the most and least efective disinfectants against P. aeruginosa, respectively. The addition of EDTA signifcantly increased the efectiveness of the selected disinfectants. The changes in the antibiotic-resistance profles after exposure to sub-inhibitory concentrations of disinfectants were observed for diferent classes of antibiotics (Carbapenems, Aminoglycosides, Cephalosporins, Fluoroquinolones). As well as near the all isolates harbored efux pump genes and 117 (97.5%) of isolates produced bioflm. Conclusion: In the current study, the mixture of disinfectant and EDTA were the most suitable selection to disinfect Hospital surfaces and instruments. Also, it was clear that exposure to sub-inhibitory concentrations of Sodium hypochlorite results in resistance to some antibiotics in P. aeruginosa species. Strong and intermediate bioflm formers belonged to MDR/XDR strains. Future studies should include more complex microbial communities residing in the Hospitals, and more disinfectants use in Hospitals. Keywords: Nosocomial infection, Disinfectant-resistance, Bioflm, Hospital disinfectants, Pseudomonas aeruginosa, Clinical isolates

Item Type: Article
Subjects: Q Science > QR Microbiology
Divisions: University Portal > research center > mmrc
Depositing User: mmrc research portal
Date Deposited: 18 May 2022 06:52
Last Modified: 18 May 2022 06:52
URI: http://eprints.qums.ac.ir/id/eprint/11520

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