Study the Effect of Au-Nanorod Conjugated With P28 Peptide on Breast Cancer Cell Line Model (Mcf-7) Using Hyperthermia

Ahmadpour Yazdi, Hosein and Salavati Nezhad, Niloofar (2021) Study the Effect of Au-Nanorod Conjugated With P28 Peptide on Breast Cancer Cell Line Model (Mcf-7) Using Hyperthermia. Masters thesis, Qazvin University of Medical Sciences, Qazvin, Iran.

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Abstract: Background and Aim: Breast cancer, as the most common cause of women’s death in the world, has different treatment methods that are not specific and targeted. Recently, hyperthermia has received much attention as one of the auxiliary methods of cancer treatment, which locally causes a controlled increase in tumor temperature (in the range of 43-49 ℃). Nowadays, the use of gold nanoparticles with biological biomolecule coating without side effects seems absolutely necessary for the faster transfer and distribution of heat in the cells, which specifically enters the cancer cell. Therefore, in this study, the therapeutic effect of hyperthermia on breast cell lines was evaluated using gold nanorods coated with polyethylene glycol and P28 peptide (derived from the azurin protein of Pseudomonas aeruginosa). Materials and methods: In this research, gold nanorods with absorption peaks of 810 and 507 nm were synthesized by seedless growth method and coated with polyethylene glycol and p28 peptide, and their characteristics were determined using spectrophotometric spectra (UV-VIS), Fourier transform to infrared ( FTIR), dynamic light scattering (DLS), transmission electron microscopy (TEM) images were confirmed. Their stability and viability were checked by MTT test. Then, using infrared laser radiation (Hyperthermia Laser) with wavelength (808 nm) and continuously for 1 minute, the therapeutic effect of this complex on breast cancer cells (MCF-7) and normal breast cells (MCF-10A) It was evaluated in vitro. Results: The obtained results indicated the synthesis of gold nanorods with the size of (4.36±25.31)*(0.88±4.52) and its conjugated with polylinker and P28 peptide, respectively. The results of laser hyperthermia on cell lines showed that the complex (GNR-PEG-P28) along with laser light radiation caused the death of 50% of breast cancer cells (MCF-7) compared to the normal line (MCF-10A). Conclusion: The results of this study showed the targeted treatment of breast cancer using nanorods coated with P28 peptide and hyperthermia method for 1 minute. In this method, due to the targeting of the complex, the amount of damage to healthy cells is greatly reduced. Key words hyperthermia, P28 peptide, breast cancer, MCF-7, NIR

Item Type: Thesis (Masters)
Subjects: R Medicine > RP Paramedical > RP105 Biotechnology
Divisions: University Thesis > Faculty of Paramedicals > Biotechnology
Depositing User: Paramedicals School Students
Date Deposited: 21 Sep 2022 05:06
Last Modified: 21 Sep 2022 05:06
URI: http://eprints.qums.ac.ir/id/eprint/11876

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