Ghayaz, Fatemeh and Zeynali kelishomi, Fatemeh and Amereh, Samira and Aali, Ehsan and Javadi, Amir and Peymani, Amir and Nikkhahi, Farhad (2023) In Vitro Activity of Fosfomycin on Multidrug‑Resistant Strains of Klebsiella pneumoniae and Klebsiella oxytoca Causing Urinary Tract Infection. Current Microbiology.
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Abstract
With the emergence of multi-drug resistant strains among Klebsiella isolates, the use of old drugs such as fosfomycin has been considered. In this context, we investigated the efect of fosfomycin on bioflm-producing Klebsiella pneumoniae and Klebsiella oxytoca strains isolated from ICU patients. A total of 90 isolates of Klebsiella pneumoniae and 30 isolates of Klebsiella oxytoca were collected from the ICU ward. All isolates were confrmed by biochemical and genotypic methods. Antibiotic susceptibility testing was performed by disc difusion method and for fosfomycin and colistin, minimum inhibitory concentration (MIC) was done using micro broth dilution. The presence of the beta-lactamase encoding genes, bioflmrelated genes, and fosfomycin resistance-related genes was detected by PCR. Finally, for fosfomycin-resistant isolates, we determined the sequence type by the MLST method. Sensitivity rate to fosfomycin in Klebsiella pneumoniae and Klebsiella oxytoca isolates was 92.2% and 100%, respectively. Fosfomycin was the most active antimicrobial agent with 96% sensitivity among all tested antibiotics. All tested isolates could produce bioflm. The frequency of bioflm-related genes for Klebsiella pneumoniae was as follows: 95.5% fmH, 86.6% mrkD, 77.7% mrkA, and 50% wcaG. The frequency of these genes for Klebsiella oxytoca was as follows: 56.6% fmA, 46.6% mrkA, 93.3% matB, and 90% pilQ. Only 4.4% of Klebsiella pneumoniae isolates showed resistance to fosfomycin, and the fosA gene was detected in all of them. Our results showed that fosfomycin efectively inhibits multidrug-resistant (MDR) strains of Klebsiella pneumoniae and Klebsiella oxytoca.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QR Microbiology |
| Divisions: | University Portal > research center > mmrc |
| Depositing User: | mmrc research portal |
| Date Deposited: | 27 Feb 2023 05:15 |
| Last Modified: | 27 Feb 2023 05:15 |
| URI: | http://eprints.qums.ac.ir/id/eprint/12317 |
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