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The effect of myricetin on the expression of b-catenin gene in melanoma cells (A375)

Abdolmaleki, fereshte and Ahmad Pour -yazdi, Hossein and Gheibi, Dr. Nematollah (2016) The effect of myricetin on the expression of b-catenin gene in melanoma cells (A375). Masters thesis, qazvin university of medical science.

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Wnt/β-catenin signaling in melanoma: Preclinical rationale and novel therapeutic insights. Cancer Treatment Reviews. 2016;12,-49:1. 169. Chien AJ, Moore EC, Lonsdorf AS, Kulikauskas RM, Rothberg BG, Berger AJ, et al. Activated Wnt/ß-catenin signaling in melanoma is associated with decreased proliferation in patient tumors and a murine melanoma model. Proceedings of the National Academy of Sciences. 2009;106(4):8,-1193. 170. Arozarena I, Bischof H, Gilby D, Belloni B, Dummer R, Wellbrock C. In melanoma, beta-catenin is a suppressor of invasion. Oncogene. 2011;30(45):43-4531. The evaluation of myricetin on the expression of β-catenin gene in melanoma cells (A375) Abstract: Background: cancer as one of the common diseases in the world, despite improving in the knowledge is expanding. Melanoma, a kind of malignant skin cancer, has high rate of fatality. Nowadays, because of chemical therapeutic side effects, natural products have attracted the attention of researches. Flavonoids, rich in plants and vegetables, are polyphenol compound with low molecular weight which have chemopreventive properties such as anti-cancer, anti-oxidant, anti- angiogenesis, and anti- inflammatory effect. Between different classes of flavonoids, myricetin due to its structure (hydroxyl groups and C=C bound) include more specifities. Wnt signaling pathway, a signaling pathway in melanoma cells contributes in initiation, proliferation, and differentiation. β-catenin as a key factor in this pathway could be considered a therapy target. Objective: This study aimed to evaluate the effect of myricetin flavonoid compound on the expression of β-catenin in melanoma cells (A375) Methods: In this study, the effect of myricetin on the expression β-catenin in melanoma cells (A375) was determined. So the cells were treated with different concentration of myricetin (0-100), to determine myricetin IC50 by MTT assay. After finding the best concentrations, the cells were treated with different concentration (40, and 60 µM) for 48 hour. After determined time, RNA was extracted, cDNA was synthetized and real time qPCR was done. Results: The results of MTT with different concentration (0 to 100 µM), showed that myricetin IC50 for three periods (24, 48, and 72 h) were 50, 40, and 35 µM respectively. Also we detected that up to 25 µM, myricetin has no toxicity on cells. So in order to determine the expression of β-catein, the cells were treated with 40, and 60 µM myricetin. The result of real time qPCR showed the expression of β-catenin gene has increased, so that following higher concentration of myricetin treatment, a moderate increment of β-catenin gene expression is seen. Conclusion: myricetin has anti- proliferative potential. Since A375 cells are malignant and metastatic, so the expression of β-catenin is spontaneously low, thus myricetin with inhibitory activity could increase the β-catenin expression and suppress the melanoma cells towards being metastatic. Suggestion: scine this study has been effective in invitro situation, it is necessary to search the effect of this product in invivo to acknowledge the inhibitory effect of myricetin. Also in order to enhance the half life of this product to be used along with other treatments, it is recommended to use it with nanoparticle. On the other hand, to determine the metastatic potential of A375 cells and the inhibitory effect of myricetin on the β-catenin expression, it is recommended 1. Determine the expression of β-catenin protein by Western blotting 2. Examine this flavonoid on other metastatic cells to be sure of its inhibitory effect, and 3. Determine the metastic potential of A375 cells based on the level of adherance.

Item Type: Thesis (Masters)
Subjects: R Medicine > RH Basic Medical Sciences
R Medicine > RP Paramedical > RP105 Biotechnology
Divisions: University Thesis > Faculty of Paramedicals > Biotechnology
Depositing User: Paramedicals School Students
Date Deposited: 04 Oct 2016 08:25
Last Modified: 14 Jan 2017 08:17
URI: http://eprints.qums.ac.ir/id/eprint/5186

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