qums

Analysis of effects of Omega-3, -6 and -9 fatty acids on expression of ErK protein relating to MAPK signaling pathway in SK-OV-3 cell line, relating to ovarian cancer

Hajighasemi, Saeideh and Gheibi, Dr. Nematollah and Karimipour, Dr.Morteza and Azad, Dr. Mehdi (2016) Analysis of effects of Omega-3, -6 and -9 fatty acids on expression of ErK protein relating to MAPK signaling pathway in SK-OV-3 cell line, relating to ovarian cancer. Masters thesis, Qazvin Univerity of Medical Sciences.

[img] PDF
Download (254kB)

Abstract

Despite recent progress in cancer treatment, cancer still remains as a major public health problem in many parts of the world. Epithelial ovarian cancer is one of the most mortal and treat-resistant cancers among gynecological cancers. A novel approach to chemotherapy has the potential to yield novel dietary-drug, dietary-dietary and/or drug-drug combinations that can provide additive or even synergistic protection against the progression of cancers. There is considerable evidence that unsaturated fatty acids, in addition to their role as an energy source, might affect both cancer development and progression. In this study we investigated cytotoxic effects of n-3, n-6 and n-9 unsaturated fatty acids on SKOV-3 (human ovarian adenocarcinoma) cell line by MTT assay. Using an Annexin-V-FLOUS staining kit, we evaluated apoptosis induction in these cells. Finally, we performed western blotting for ErK and phospho-ErK in order to investigate the impact of unsaturated fatty acids on MAPK signaling pathway. The MTT results showed that Linoleic acid (LA, n-6) and Oleic acid (OA, n-9) at concentrations lower than 500 µM promoted cell proliferation and were cytotoxic at higher concentrations. On the other hand, Alpha-linolenic (ALA, n-3) acid and Arachidonic acid (AA, n-6) had no effect on SKOV-3 cell proliferation at concentrations used. Also, it has been clarified cytotoxic effects of LA and OA is due to their ability to induce apoptosis. Following western blotting it has been determined that LA and OA reduced ErK activation.

Item Type: Thesis (Masters)
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
R Medicine > RG Gynecology and obstetrics
R Medicine > RP Paramedical > RP105 Biotechnology
Divisions: University Thesis > Faculty of Paramedicals > Biotechnology
Depositing User: Paramedicals School Students
Date Deposited: 06 Dec 2016 10:25
Last Modified: 06 Dec 2016 10:32
URI: http://eprints.qums.ac.ir/id/eprint/5223

Actions (login required)

View Item View Item