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Effect of Methotrexate on the structure and stability of human serum albumin (HSA

Hashemie, zohreh (1396) Effect of Methotrexate on the structure and stability of human serum albumin (HSA. Masters thesis, qazvin university of medical sciences/qazvin/iran.

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Abstract

Human serum albumin (HSA) as the most abundant carrier protein in blood plasma has been widely studied. HSA is often used as a model to study of the drug and protein interaction. Basically, studying the interaction between the drug molecules and HSA helps to understand the transportation, distribution and metabolism of the drug in human body, and also has a very important significance for clarifying its pharmacological effects and pharmacokinetics. The interaction between Methotrexate with HSA under simulative physiological conditions has been studied by methods such as fluorescence.Thermodynamic parameters obtained from thermal and chemical denaturation of the Human serum albumin with and without the presence of Methotrexate was analyzed. Tm values in melting point (Tm) of HSA in the absence of these compounds were calculated 320/9 K and in the presence of Methotrexate(50μM) were obtained 318/4 K and the presence of Methotrexate(100μM) were obtained 318/5K, respectively .ΔGo (298k) of HSA in the absence and presence of Methotrexate 50 and 100 were measured 41.14, 39/4 , 15/2 respectively. Chemical denaturation of HSA carried out with urea and titrated amounts of the protein in 280 nm, ΔGo(H2O) and Cm values were calculated in the absence of these compounds ΔGo(H2O) was calculated 13/43 kJ/mol and in the presence of Methotrexate(50μM) was calculated 11/63 and Methotrexate(100μM) was calculated 11/1 kJ/mol in the presence of Methotrexate respectively. Cm value of the protein in the absence of Methotrexate s was 1/57 M and in the presence of Methotrexate (50μM) were 2/06 M and Methotrexate(100 μM) 2/03M, respectively. Intrinsic fluorescence studies showed that Methotrexate decrease the intrinsic fluorescence intensity at 300-450 nm which consequently results in structural instability of HSA. Keywords: Human serum albumin, Methotrexate

Item Type: Thesis (Masters)
Subjects: R Medicine > R Medicine (General)
Divisions: University Thesis > Faculty of Medicine
Depositing User: Medicine School Students
Date Deposited: 22 May 2017 09:58
Last Modified: 22 May 2017 09:58
URI: http://eprints.qums.ac.ir/id/eprint/6365

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