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Capsid Modified Bluetongue Virus 16 (BTV16) as a Virulytic Oncotherapy Agent

Naserpour Farivar, Taghi and Najafipour, Reza and Alizadeh, Safar Ali and Azimi, Seyyed Mahmoud and Johari, Pouran (2017) Capsid Modified Bluetongue Virus 16 (BTV16) as a Virulytic Oncotherapy Agent. Biotechnology and Health Sciences, S (1). pp. 41-46. ISSN 2383-028x

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Abstract

Objective: Using potential viruses to destroy cancer cells has a long history, but recent advances in molecular biology raised hopes for successful use of these viruses again. Methods: Octreotate sequence was inserted into the neutralization region (R1& R2) in vp2 protein of capsid segment in 10 segmented genome of BTV in 304 - 368 position. T7 BTV RNA transcripts were extracted. Cancerous cultured cells were transfected with wild and modified BTV to recover BTV with cDNA-derived genome segments. Results: The results of all the performed experiments revealed that treatment of AGS cell lines with VP2 modified BTV16, which targeted cell surface of cancerous cells, significantly increased apoptosis in cancer infected cells. Conclusions: Modified VP2 BTV16 may be used as a potential virulytic oncotherapy agent in AGS cells. Keywords: Bluetongue Virus, Oncotherapy, Virulytic, AGS

Item Type: Article
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: University Portal > vice chancellor > vcr
Depositing User: pr vcen research
Date Deposited: 27 May 2017 06:53
Last Modified: 27 May 2017 06:53
URI: http://eprints.qums.ac.ir/id/eprint/6456

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