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Study of Omega-3 PUFA effect on expression and methylation status of WT1 & WIF1 genes promoter in pancreatic cancer cell line MiaPacaII

Rahmani, babak and Gheibi, Dr. Nematollah and sahmani, mehdi and Azad, Dr.Mehdi and hamedi Asl, Dariush and Naserpour Farivar, Taghi (2017) Study of Omega-3 PUFA effect on expression and methylation status of WT1 & WIF1 genes promoter in pancreatic cancer cell line MiaPacaII. ["eprint_fieldopt_thesis_type_phd" not defined] thesis, qazvin university of medical sciences.

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Abstract

Introduction: Anticancer effects of Omega-3 PUFAs have shown in many cancers. In cancers, signaling pathways are corrupted. These affect expression of some oncogene or tumor suppressor gene. These alterations may be due to genetic or epigenetic mechanisms such as mutation or promoter methylation respectively. One of the important signaling pathways which can be altered in pancreatic cancer, is Wnt signaling pathway Aims: This study for first, investigated omega-3 PUFA (ALA) effects on expression and promoter methylation of WT1 and WIF1 genes, two key genes of Wnt pathway. Materials and methods: Pancreatic cell line MiaPacaII, were cultured in 96 and 6 well plates for MTT and expression analysis respectively. Omega-3 PUFA in 25, 50, 100, 250, 500 and 1000 µM were prepared and then introduced to each wells. Cells harvested after 24, 48 and 72 hrs and assesed for expression, methylation and proliferation effects of each concentration separately. Results: Data obtained from analysis showed that Omega-3 with 25 and 50 µM concentrations has no significant effecte on both WIF1 and WT1 gene expression after 24, 48 and 72 hrs. In addition, 100, 250, 500 and 1000 µM had significant effect on both WIF1 and WT1 gene expression after 24, 48 and 72 hrs. WIF1 gene was upregulated and WT1 gene was downregulated due to concerned concentrations. MTT assay showed antiproliferative effects for omega-3 on 100, 250, 500 and 1000 µM but not 25 and 50 µM. None of examined concentrations had significant effect on WT1 and WIF1 gene promoter methylation. Coclussions: Omega-3 PUFA (ALA) can act as an antiproliferative supplement and asserts this effect via altering gene expression pattern. Many genes are regulate by different concentrations of omega-3 and between these are WIF1 and WT1 genes. It is possible that ALA do not have effect on promoter methylation of WIF1 and WT1 genes. Key Words: Omega-3, MiaPacaII, Wnt signaling, WIF1, WT1, Paccreatic cancer, methylation

Item Type: Thesis (["eprint_fieldopt_thesis_type_phd" not defined])
Subjects: R Medicine > RH Basic Medical Sciences
Divisions: UNSPECIFIED
Depositing User: babak rahmani
Date Deposited: 04 Nov 2017 06:38
Last Modified: 04 Nov 2017 06:38
URI: http://eprints.qums.ac.ir/id/eprint/6892

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