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Distribution of the blaOXA, blaVEB-1, and blaGES-1 genes and resistance patterns of ESBL-producing Pseudomonas aeruginosa isolated from hospitals in Tehran and Qazvin, Iran

Amirkamali, Sahar and Naserpour Farivar, Taghi and Azarhoosh, Khadijeh and Peymani, Dr. Amir Distribution of the blaOXA, blaVEB-1, and blaGES-1 genes and resistance patterns of ESBL-producing Pseudomonas aeruginosa isolated from hospitals in Tehran and Qazvin, Iran. Revista da Sociedade Brasileira de Medicina Tropical.

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Abstract

Introduction: Pseudomonas aeruginosa is one of the most common nosocomial pathogens. The emergence of extended spectrum β-lactamases (ESBLs) has been increasingly reported as a major clinical concern worldwide. The main aim of the present study was to determine the distribution of blaOXA, blaPER-1, blaVEB-1, and blaGES-1 genes among ESBL-producing P. aeruginosa isolated from two distinct provinces in Iran. Methods: In this study, a total of 75 (27.5%) ESBL-producing isolates were identified from 273 P. aeruginosa isolates collected from patients in Qazvin and Tehran. Phenotypic detection of ESBLs and antimicrobial susceptibility testing were performed according to the Clinical and Laboratory Standards Institute guidelines. PCR and sequencing were employed to detect blaOXA-1, blaOXA, blaGES-1, blaPER-1, and blaVEB-1 genes. Isolate genetic relationships were evaluated by repetitive extragenic palindromic sequence-based PCR (REP-PCR). Results: In total, 59 (78.7%) of the ESBLproducing isolates showed multidrug resistance. The highest rates of susceptibility were observed against colistin (75 isolates, 100%) and polymyxin B (75, 100%) followed by amikacin (44, 58.7%), and piperacillin-tazobactam (40, 53.3%). The blaOXA-1 (37.3%) gene was the most common of the genes investigated, followed by blaOXA-4 (32%), blaGES-1 (16%), and blaVEB-1 (13.3%). REP-PCR identified three different genotypes: types A (89.3%), B (6.7%), and C (4%). Conclusions: We found a significant presence of blaOXA-1, blaOXA-4, blaGES-1, and blaVEB-1 genes among P. aeruginosa isolates, highlighting the need for suitable infection control strategies to effectively treat patients and prevent the further distribution of these resistant organisms.

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Divisions: University Portal > research center > mmrc
Depositing User: pr mmrc research portal
Date Deposited: 26 Dec 2017 04:01
Last Modified: 26 Dec 2017 04:01
URI: http://eprints.qums.ac.ir/id/eprint/6979

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