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Biodegradation of Endosulfan as an Organochlorine Pesticide with Pseudomonas plecoglocissida Transfected by LinA Gene

Aslanimehr, Masoumeh and Naserpour Farivar, Taghi and Najafipour, Reza and Peymani, Dr. Amir and Alizadeh, Safar Ali and Johari, Pouran Biodegradation of Endosulfan as an Organochlorine Pesticide with Pseudomonas plecoglocissida Transfected by LinA Gene. Biotechnology and Health Sciences.

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Abstract

Background: Organochlorine pesticides (OC) are applied to soil and agricultural farms and can contaminate sewerage and ditches, which in turn may be transported to rivers, and consequently distributed in the environment and as a pollutant that endangers human health. Objectives: This study aimed at constructing a genetically engineered Pseudomonas plecoglossicida strain enabled to degrade the OC pesticide, endosulfan. Methods: Transfected Pseudomonas plecoglossicida strain was initially made in the following steps, first polymerase chain reaction (PCR) product of gene LinA from Sphingomonas paucimobilis UT26 was transferred to the pUC57 plasmid, and the plasmid was then transfected to P. plecoglossicida. Results: In endosulfan supplemented carbon-deficient minimal medium, the isolate showed its ability to hydrolyze endosulfan to endosulfan sulphate. The isolatewasobserved togrowonendosulfan as the carbon source. Thus, the strainwascapable of degrading the OC pesticides and utilizing products of their degradation as nutrients. Conclusions: Ability of transfected Pseudomonas plecoglossicida strain in biodegradation of chemicals makes it an excellent candidate for bioremediation of contaminated agricultural and industrial sites.

Item Type: Article
Subjects: UNSPECIFIED
Divisions: University Portal > research center > mmrc
Depositing User: pr mmrc research portal
Date Deposited: 15 Oct 2018 05:04
Last Modified: 15 Oct 2018 05:04
URI: http://eprints.qums.ac.ir/id/eprint/7990

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