Kalantari, A and Naserpour Farivar, Taghi and Peymani, Amir and Aslanimehr, Masoumeh and Bagheri Nezhad, R (2017) EXTRACTION AND CHARACTERISATION OF BRUCELLA ABORTUS STRAIN RB51 ROUGH LIPOPOLYSACCHARIDE. Bulgarian Journal of Veterinary Medicine.

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Brucellosis is an important zoonotic disease with considerable impacts on human and animal health. Brucella abortus strain RB51 vaccine is used for prevention of bovine brucellosis in Iran. Due to strain roughness, available serological tests cannot detect vaccinated animals. Detection of serological responses to the vaccine is important to monitor accurate vaccination implementation. Rough lipopolysaccharide (RLPS) of RB51 strain was extracted and characterised to develop serological tests for diagnosis of vaccinated animals. RLPS was extracted using phenol-chloroform-petroleum ether and evaluated by limulus amebocyte lysate (LAL) assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and agar gel immunodiffusion (AGID). According to our results, the extracted RLPS caused positive reaction in LAL assay. In SDS-PAGE, a band with a molecular weight around 14 kDa was identified after specific staining using silver nitrate. Double AGID of the RLPS with a hyperimmune serum resulted in a precipitation line formation. Our study showed that the method can be successfully used to extract RLPS from Brucella abortus strain RB51 as confirmed by LAL assay, PAGE and AGID. Key words: brucellosis, RB51 vaccine, rough lipopolysaccharide

Item Type: Article
Subjects: Q Science > QR Microbiology
Divisions: University Portal > research center > camr
Depositing User: pr Camr research
Date Deposited: 24 Sep 2019 05:48
Last Modified: 24 Sep 2019 05:48
URI: http://eprints.qums.ac.ir/id/eprint/8887

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