Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker

Shahnazi, Mojtaba and Zarezadeh Mehrizi1, Majid and Alizadeh, Safarali and Heydarian, Peyman and Saraei, Mehrzad and Alipour, Mahmood and Hajialilo, Elham (2019) Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker. African Health Sciences.

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Abstract Introduction: Hymenolepis nana is a zoonotic tapeworm with widespread distribution. The goal of the present study was to identify the parasite in the specimens collected from NorthWestern regions of Iran using PCR-sequencing method. Methods: A total of 1521 stool samples were collected from the study individuals. Initially, the identification of hymenolepis nana was confirmed by parasitological method including direct wet-mount and formalin-ethyl acetate concentration methods. Afterward, PCR-sequencing analysis of ribosomal ITS2 fragment was targeted to investigate the molecular identification of the parasite. Results: Overall, 0.65% (10/1521) of the isolates were contaminated with H. nana in formalin-ethyl acetate concentration. All ten isolates were succefully amplified by PCR and further sequenced. The determined sequences were deposited in GenBank under the accession numbers MH337810 -MH337819. Conclusion: Our results clarified the presence of H. nana among the patients in the study areas. In addition, the molecular technique could be accessible when the human eggs are the only sources available to identify and diagnose the parasite. Keywords: Hymenolepis nana, rDNAITS2, PCR, Iran

Item Type: Article
Subjects: R Medicine > RQ Parasitology
Divisions: University Portal > research center > camr
Depositing User: pr Camr research
Date Deposited: 03 Oct 2019 08:05
Last Modified: 03 Oct 2019 08:05
URI: http://eprints.qums.ac.ir/id/eprint/9042

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