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The effects of omega-3 PUFA (ALA) on WT1 gene expression in pancreatic cancer cell line (MIA PaCa-2)

Rahmani, babak and Hamedi, Dariush and Naserpour Farivar, Taghi and Azad, Mehdi and sahmani, mehdi and Gheibi, Nematollah The effects of omega-3 PUFA (ALA) on WT1 gene expression in pancreatic cancer cell line (MIA PaCa-2). BASIC RESEARCH.

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Abstract

Background: Cancer starts by cells acquiring a number of characteristic alterations especially abnormal cell growth. These properties reflect faults in signalling pathways in cancer cells. Wilms’ tumor 1(WT1) acts as a tumor suppressor by negative regulation of WNT/beta-catenin signaling pathway. The role of WT1 in many cancers has been studied. Aims: To evaluate the WT1 gene expression alterations in response to different concentrations of omega-3 PUFA alpha linolenic acid (ALA) in pancreatic cancer cell line (MIA PaCa-2) in a time dependent manner. Methods: MiaPaca-2cell line was cultured in monolayers. After that began the cells treatment with omega-3 fatty acid (ALA) using different concentrations of 25, 50, 100, 250, 500 and 1000 μM for 24, 48 and 72 hours. The RNA was extracted from both control (untreated) and treated cells. Viability was checked by MTT assay and WT1 expression was evaluated by RT-PCR. Results: Real-time RT-PCR analysis showed that the level of WT1 mRNA, was decreased after 24, 48, 72 hours treatment with omega-3 fatty acid (ALA) for 100, 250, 500 and 1000 μM concentrations but not 25 and 50 μM. Data obtained from MTT revealed antiproliferative effects of omega-3 fatty acid (ALA) for 100-1000 μM concentrations but not 25 and 50 μM. Conclusion: Our findings indicated that the cell viability and level of WT1 mRNA was decreased during Omega 3 ALA Treatment. So, it a potential role of Omega 3 in prevention or as a supplementary for pancreatic cancer treatment has been suggested. Key words: omega-3 PUFA (ALA), pancreatic cancer, MIA PaCa-2, WT1

Item Type: Article
Subjects: R Medicine > RH Basic Medical Sciences
Divisions: University Portal > research center > camr
Depositing User: pr Camr research
Date Deposited: 21 Oct 2019 06:20
Last Modified: 21 Oct 2019 06:20
URI: http://eprints.qums.ac.ir/id/eprint/9049

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