Comparison of real-time PCR and cultural method for detection of bacterial load in pasteurized milk

Farhoudi, Ayda and Ghajarbeygi, Peyman and Jafari Jozani, Razi Allah and Mahmoudi, Razzagh and Mardani, Karim (2019) Comparison of real-time PCR and cultural method for detection of bacterial load in pasteurized milk. Journal of Food Safety, 39 (3). pp. 1-5.

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Abstract

In this study, we used a TaqMan® probes through the real-time polymerase chain reaction (PCR) technique as a rapid and sensitive way to detect bacterial load of pasteurized milk. The reaction was optimized for enumeration of bacteria in pasteurized milk samples. In parallel, the same milk samples were assessed by conventional cultural-based method. The correlation between methods was evaluated by Bland Altman analysis. The minimum and maximum value for conventional culture-based method were 0 and 10,000 cfu/ml while qPCR gave us 55 and 7,071 (Bacteria/ml of pasteurized milk), respectively. Results indicated that Taq Man real-time PCR provides a useful tool for rapid and accurate quantification of bacterial load.

Item Type:	Article
Subjects:	Q Science > QR Microbiology R Medicine > RN Health > RN1006 Health and food safety
Divisions:	University Portal > research center > hpsr
Depositing User:	pr hpsr research center
Date Deposited:	15 Jul 2020 05:29
Last Modified:	15 Jul 2020 05:29
URI:	http://eprints.qums.ac.ir/id/eprint/9852

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